BIOB10H3 Study Guide - Ultraviolet, Green Fluorescent Protein, Dichroic Filter

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Techniques in cell and molecular biology: microscopes are instruments that produce enlarged image of an object. A light source, which illuminates the specimen, is critical within the microscope. Condenser lens gathers the diffuse ray from the light source and illuminates the specimen with a small cone of bright light that allows very small parts of the specimen to be seen after magnification. Light rays focused on the specimen by the condenser lens are then collected by the microscope"s objective lens. Two sets of light rays that enter objective lens: those that the specimen had altered and those that it hasn"t. Image formed by the objective lens is used as an object by a second lens system, the ocular lens, to form an enlarged and virtual image. If you were to switch from the 5x to 10x ocular, you would most likely increase your ability to determine the number of details present within cell.

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