BIOD60H3 Study Guide - Lipid Bilayer, Lysosome, Column Chromatography
FSG Session #5: BIOB10Y3: Cell and Molecular Aspects of Genetic Processes
Section # 1: Terms to know
_______________ _____________ : A membrane-associated protein that is located
entirely outside of the lipid bilayer and interacts with it through noncovalent bonds.
_________________ protein : A membrane-associated protein that is located outside the
bilayer but is covalently linked to a ________ molecule within the _________.
Section # 2: Short Answers
1) What is Receptor mediated endocytosis (REM)? Describe the steps involved.
2) What are some functions of the Plasma membrane?
3) The main types of membrane lipids are? What are some defining characteristics of each type?
4) How does the Sodium-Potassium Pump function? What kind of transport is this?
5) A molecule normally enters a cell by simple diffusion through the lipid bilayer. What happens
if you artificially reverse the molecule's concentration gradient?
6) What does the protein purification process involve? Describe the Cell Fractionation steps.
7) What would an Ab made against goat-actin in a mouse called?
8) Describe to someone the 3 different column chromatography separation techniques that were
introduced in lecture.
9) Which technique effectively separates proteins when they travel through a charged medium, to
which some of the proteins adhere, while others do not adhere and pass through the column.
Section # 3: Multiple Choice
1) In what order are proteins eluted from an ion exchange column?
a. Proteins are eluted from the column in order from the least strongly bound to the most strongly bound.
b. Proteins are eluted from the column in order from the most strongly bound to the least strongly bound.
c. Proteins are eluted from the column in order from the least denatured to the most denatured.
d. Proteins are eluted from the column in order from the most denatured to the least denatured.
e. Proteins are eluted from the column in order from the least stable to the most stable.
2) A channel that opens in response to the binding of a specific molecule, which is usually not the solute that passes through
the channel is called a ________.
a. voltage-gated channel
b. charge-gated channel
c. ligand-gated channel
d. positive-gated channel
e. electric-gated channel
3) What directly or indirectly determines the transition temperature?
a. the ability of lipid molecules to be packed together
b. whether the fatty acid chains of the lipids are saturated or unsaturated
c. the extent to which the fatty acid chains of the lipids contain double bonds
d. the length of the fatty acid chains
e. all of the above
4) The most stable atoms and thus those that are typically nonreactive are the atoms that have __?
a. equal numbers of electrons and protons
b. equal numbers of electrons and neutrons
c. full inner shells
d. full outer shells
e. all covalent bonds
5) What level of structure in proteins is held together by intermolecular R group interactions?
a. primary structure
b. secondary structure
c. tertiary structure
d. quaternary structure
6) Which of the proteins below is(are) not made on the membrane-bound ribosomes of the RER?
a. peripheral membrane proteins
b. soluble lysosomal proteins
c. vacuolar enzymes
d. proteins of the extracellular matrix
e. all of the above
7) Which coated vesicles move materials in a retrograde direction from the ERGIC and Golgi stack toward the
a. COPII-coated vesicles
b. COPI-coated vesicles
c. clathrin-coated vesicles
d. cadmium-coated vesicles
e. a and b
8) What is responsible for recognizing lysosomal enzymes and localizing them to the lysosomes?
a. mannose 6-phosphate receptors
c. integral membrane proteins that span the TGN membranes
d. all other answers are correct