bioa02.formal lab report.docx

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Biological Sciences
Ivana Stehlik

Comparison of different effects of nutrients on Barley (Hordeum vulgare) and (Secale cereal). Student number: Practical section: 00 Teacher Assistant’s stme: Urja Naik Date submitted: April 1 2013 Page 1 out of 11 Abstract A full complement nitrogen inclusive solution helps improve plant growth as well as absorption of more nitrogen nutrients. The species barley (Hordeum vulgare) and Rye (Secale cereal) plant are crop plants that show increased mean seedling shoot growth. The more nutrient a plant receives the more the plant develops. The important nutrient nitrogen should be added to crop plants to boost plant growth and nitrogen retention. The macronutrient nitrogen is kept in plants even under stressful environments and shows more than 5% longer shoot growth rate compared to the growth rate of nitrogen deficient barley (Hordeum vulgare) and Rye (Secale cereal) plant. It is significant to take note of the benefits nitrogen inclusive treatment brings to plant and recommend its introduction to more plants in order to improve plant growth. Introduction Nitrogen is abundantly found in the environment, and plays a mandatory role in plants because it is a macronutrient. How does the nutrient affect plant growth? In the conducted experiment, barley (Hordeum vulgare) and Rye (Secale cereal) plant species are subjected to three different treatments: age water (no added nutrient), nitrogen exclusive nutrient, and nitrogen inclusive nutrient. The shoot length and weight are measure in order to determine how under different nutrient system will plant growths be affected. It is important to know if the nutrient will play a positive or negative role to the plant in order to recommend treatment types to plants. Would age water, nitrogen inclusive, or nitrogen exclusive solution be best Page 2 out of 11 treatment for the crop plants barley (Hordeum vulgare) and Rye (Secale cereal) plant? Nitrogen is used in lateral plant growth (Drew, 1975). This would be a positive factor towards plant growth because it could increase water uptake and expand surface area for carbon fixation. If there were an increase of nutrients in the plant, then plant growth would increase. This is because with more macronutrients such as Carbon, Oxygen, Hydrogen, Nitrogen, Sulfur, Phosphorus, Potassium, Calcium, Magnesium, plants would receive more essential elements for plant growth. Since Nitrogen is needed in larger amounts, it is recommended full complement nutrients should be added into crop plants in order to help develop plant growth. Methods The materials used include: Barley (Hordeum vulgare), Rye (Secale cereal), paper towel, plastic container, cotton, fluorescent light, marker, ruler, excel spreadsheet, data table, treatments (solutions of full nutrient complement, N-deficient, no added nutrient). Cut one paper towel in half, fold and place on bottom of container and place a layer of cotton on top of the paper towel, and followed by another layer of paper towel. Add 30 ml of one of the solution to the container and allow the solution to absorb, spread 10 ml of Hordeum vulgare or Secale cereal onto the paper towel, fold a layer of paper towel on top of the seed, add 20 ml of the same solution, and place container under fluorescent light. After 14 days, remove the seed from container and measure roots using a ruler, and weigh seed. Write results onto data table and enter data into the Excel spreadsheet. Page 3 out of 11 Results Mean seedling weight vs Seed Type No Nitrogen Complete medium Mean seedling weight (g) Seed Type Barley (Hordeum vulgare) and Rye (Secale cereal) Figure 1 Relationship between mean seedling weight (g) and Seed Type Barley (Hordeum vulgare) (t=4.24,df=22,p<0.05) on the left two columns, and Rye (Secale cereal) (t=5.36,df=22,p<0.05) on the right two columns. Page 4 out of 11 Mean seedling shoot length vs Seed Type No Nitrogen Complete medium Mean seedling shoot length (cm) Seed Type Barley (Hordeum vulgare) and Rye (Secale cereal) Figure 2 Relationship between mean seedling shoot length (cm) and Seed type Barley (Hordeum vulgare) (t=1.53,df=22,p>0.05) on the left two columns, and Rye (Secale cereal) (t=2.00,df=22,p>0.05) on the right two columns. Figure 3 Barley (Hordeum vulgare) under (a) aged water treatment, (b) full complement N inclusive treatment, (c) N exclusive treatment. Page 5 out of 11 Table 1 Plant Seedling shoot length data for each treatment (water, No Nitrogen, complete solution), barley (Hordeum vulgare) vs. rye (Secale cereal). TEST 1 TEST 2 TEST 3 Rye Barley Rye No Rye TREATMENT Barley Water No N N Barley Complete Water Complete 12 12 12 12 12 12 Sample Size (n) 17.40 14.21 19.22 16.49 20.75 20.94 Mean (x) Standard Deviation 2.69 1.96 3.80 6.19 4.25 4.60 (s) Variance (s ) 7.22 3.83 14.48 38.37 18.08 21.20 0.78 0.56 1.10 1.79 1.23 1.33 SEM (√ s / n) 3.32 0.07 0.10 Sample t Statistic Mean -1Mean 2 2 2 √ SEM 1 + SEM 2 Degrees of Freedom df =22 df =22 df =22 df = (1 + n2) - 2 Critical t – value:2.086 Critical t – value: Critical t – value: 2.086 2.086 Critical t Statistic P value: p>0.05 P value: p>0.05 from t -Test Table P value: p<0.05 for two-tail test and p
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