BIOB10H3 Study Guide - Midterm Guide: Photobleaching, Epitope, Cyan
8 Dec 2017
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Fluorescence microscopy immuno uorescence: gfp technology uses uorescence microscope detect location of uorophores. Fluorophores or uorescent dyes absorb energy of speci c wavelengths release a portion of this energy as longer visible wavelengths of light called. Uorescence beam splitting mirror = bend any light of short wavelength to be absorbed in the object and allow long wavelength to be shown. Uorophore could be attached to an antibody = immuno uorescence. Ab made against goat- rab6 in a mouse: mouse anti-goat rab6 antibody. Check the animals blood for antibodies against the antigen. B cells t only one region of the antigen = epitope. B cell that will interact with each component of the antigen. Antibodies that all react against the injected protein = polyclonal antiserum comes from antibodies generated from many di erent types of b cells. Single clone of b cell produces antibodies against antigenic components = monoclonal antibodies. 1 type of b cell and 1 tumour cell.
