BIOB11H3 Study Guide - Midterm Guide: In Situ Hybridization, Nucleic Acid Hybridization, Genome Size
Document Summary
Biob11- lecture 2- genome organization and molecular evolution. Absorption spectrum: a plot of the intensity of light absorbed relative to its wavelength. Complexity: a measure of the number of unique (vs. repetitive) sequences that exist in a genome. Hybridization: 2 strands of dna together forming complementary base pairs (if they can) Renaturation (reannealing): reassociation of complementary single- stranded dna molecules that had been previously denatured. Depends on many factors like ionic strength, length of fragments, temperature, and time. In situ hybridization: technique to localize a particular dna or rna sequence in a cell or on a culture plate or electrophoretic gel. Centromere: marked indentations on a mitotic chromosome that serve as the site for kinechore formation. Telomere: an unusual stretch of repeated dna sequences, which forms a cap at each end of a chromosome. Mutation: a spontaneous change in a gene that alters it in a permanent fashion so that it causes a heritable change.
