BIOB11H3 Study Guide - Midterm Guide: Ribosomal Rna, Tata Box, Sigma Factor

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greenherring451

28 Feb 2013

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Biob11 - lecture 4 an overview of transcription, rrna synthesis and maturation. Tata box: at the position of -10 upstream from the start point, there is a conserved sequence that is found in almost every gene tataat (see upper strand 5" to 3") known as the tata box. It is where the sigma factor binds and brings the core enzyme to start transcribing at +1 (start position) Consensus sequence: the most common version of a conserved sequence. The ttgaca sequence of a bacterial promoter (known as the -35 element) is an example of a consensus sequence. Amanitin: is a toxin found in some mushrooms. The three classes of rna polymerase are classified by their susceptibility to the alpha amanitin. Ribosomes: are large complexes that consist of several ribosomal rna"s and many proteins. Nucleolus: clusters of ribosomal rna genes and site of ribosome assembly.

Related Questions

In prokaryotes, a mutation in which of the following is likely to change the position of hte transcriptional start site (the +1 position)?

genes that encode subunits of the core RNA polymerase

the Shine-Dalgarno sequnce the

TATA box

the cleavage and polyadenylation site

the -35 consensus sequence

the Rho utilization site (Rhut)

burgundydonkey451

9. The nucleotide on the DNA template that marks the starting point for RNA synthesis is called

a. 0 (zero point)

b. +1

c. -1

d. Start codon

e. TATA

10. Which bacterial RNA polymerase synthesizes tRNA?

a. RNA polymerase I

b. RNA polymerase II

c. RNA polymerase III

d. Core enzyme

e. Promoter

11. What general transcription factor has enzymatic activity?

a. Operator

b. TFIIH

c. eIF2

d. RNAP II

e. CTD

12. What enzyme activity/activities is/are known to be associated with the transcription factor in the previous question?

a. protein kinase and a DNA helicase

b. protein phosphatase

c. ribozyme

d. synthesis of RNA

e. a and c

fuchsiaclam492

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

BIOB11H3 Study Guide - Midterm Guide: Ribosomal Rna, Tata Box, Sigma Factor

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