BIOB11H3 : Chapter 14 Notes -Lecture 17 and 18 (2012)

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13 Apr 2012
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Lecture 17 cell cycle ii chromosome condensation and movement. Cytoskeleton disassembles, spindle apparatus forms; nuclear membrane breaks down. Prometaphase chromosomes are captured by mts at centromere/kinetochores; and begin movement to equator. Start: is when g1 cyclin/cdk triggers transition from g1 to s phase. Experiment showed that there is a factor in the cytoplasm that triggers cell division. This was called : maturation promoting factor (mpf) was this factor in the cytoplasm. And it was shown that the active form of mpf in m-phase has: Nuclear membrane reforms: a subunit that has cyclin-dependent kinase, cyclin subunit needed for the kinase activity. Maturation promoting factor (mpf): mitotic cyclin is a type of mpf. mitotic cyclin/ckd triggers transition from g2 to m phase. We know that during s phase: dna replication initiates, centrioles are replicated. For m phase to occur: nuclear membrane breaks down (lamins must be broken down)

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