BIO130H1 Study Guide - Quiz Guide: Dna Ligase, Uracil, Base Pair

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6 Mar 2016
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Optional Textbook Study Questions – Week 4
Tutorial:
1. The advantage to the cell of being able to package DNA into a heterochromatic state
include all of the following EXCEPT:
a. Heterochromatic AND is likely to be at the correct nuclear neighborhood for
high levels of transcription to take place -- NO TRANSCRIPTION AT
Heterochromatin!
b. Heterochromatic DNA is less likely to become tangled when sister chromatids
separate during mitosis
c. DNA strand in a heterochromatic state are less lily to become broken during
cellular processes
d. Heterochromatic DNA allows for the process of X-inactivation which helps
equalize the number of genes in human males and females
2. The leading strand is synthesized continuously from one RNA primer
1. Review. Take the “Flow of Information” diagram/flowchart that you made in the
Optional Textbook Study Question #1 from Week 1 and add some notes/pictures to
show how DNA replication fits into your diagram or flowchart (hint: in this case we’d be
thinking about information flow between cells).
2. The figure below shows an origin of replication. The arrows show the direction of lagging
strand synthesis. Using arrows to illustrate the direction of DNA synthesis, draw and label
the leading and lagging strands. Label the 5’ and 3’ ends of all newly synthesized strands
and the template strands. Indicate where all the RNA primers would be. Also label the
Okazaki fragments.
3. Use the table below as a template, or create your own table or diagram to review DNA
replication in bacteria. You may want to construct this table in such a way that you can add
to it later (you might want to compare prokaryotic DNA replication, transcription, and
translation later in the term).
Bacterial DNA Replication (synthesis)
Template (DNA or RNA) DNA
Direction of synthesis 5’--3’
Product (DNA, RNA, or protein) DNA
Start “signal” and proteins or factors
required
DNA is unwound by helicase and primase
Stop Signal XXX (not applicable here)
Enzymes or factors required for synthesis
(name of enzyme or factor plus function)
Helicase: unwind DNA
Primase: provide RNA primer
DNA polymerase: and nucleotides (dNTPs)
on to the daughter strand
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Document Summary

Take the flow of information diagram/flowchart that you made in the. The arrows show the direction of lagging strand synthesis. Using arrows to illustrate the direction of dna synthesis, draw and label the leading and lagging strands. Label the 5" and 3" ends of all newly synthesized strands and the template strands. Indicate where all the rna primers would be. Okazaki fragments: use the table below as a template, or create your own table or diagram to review dna replication in bacteria. You may want to construct this table in such a way that you can add to it later (you might want to compare prokaryotic dna replication, transcription, and translation later in the term). Enzymes or factors required for synthesis (name of enzyme or factor plus function) Dna polymerase: and nucleotides (dntps) on to the daughter strand ssbps: single stranded binding proteins, prevent separated dna from reannealing. Dna ligase: seal and stitch up okazaki fragments.

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