BIO230H1 Study Guide - Final Guide: Potassium Acetate, Paper Towel, Lysis

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10 Jan 2020
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BIO230H1 Full Course Notes
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BIO230H1 Full Course Notes
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Shake the sample: transfer to separate cells. Shake to ensure nutrient availability and to avoid cells settling at the bottom of the tube, which could kill them: put on a pair of gloves, review micropipetting procedures. Blot the inverted tube on paper towel: add lysozyme to a final concentration of 50 g/ml. Determine volume of stock solution you have to add: centrifuge 30 seconds in a microcentrifuge at maximum speed. Aspirate and discard the supernatant into a waste: use a vortex to resuspend cells in 0. 5ml of solution i, centrifuge to pellet the cells. Blot to ensure that there is none left or dilutions of the concentrations of added solutes will not be correct and the resultant nucleic acid sample will be too: solution consists of sucrose that e. coli eat. Tris-hcl is a good buffer solution for nucleic acids and keeps the ph at an ideal 8.