BIO230H1 Study Guide - Final Guide: Cell Membrane, Cell Nucleus, Peptidoglycan
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BIO230H1 Full Course Notes
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Document Summary
Rapid isolation and electrophoresis of rna from e. coli. Bacterial cells contain three main class of rna: ribosomal rna (rrna, transfer rna (trna, messenger rna (mrna, through extraction and completing electrophoresis most rna detectable on the gel is either rrna or trna. Lysozyme: disrupts cell wall by hydrolyzes the beta-glycosidic linkages that connect. Sds: lyse cells it is a strong ionic detergent that solubilizes the plasma membrane, it also is a strong protein denaturer remove protein and other cellular constituents. Potassium acetate: sds + potassium acetate is to remove proteins and other cellular constituents it prevents damage to rna. Nacl: k+ forms ionic bond to produce potassium dodecyl sulfate (insoluble) this will remove most protein of the unbroken cell. Rna it will neutralize the negative charged rna backbone decreasing the solubility of rna in water. Ethanol: has high salt solution - decreases solubility of rna causing it to precipitate.
