Lecture 29 Krebs Cycle.docx

4 Pages
Unlock Document

University of Toronto St. George
Michael Baker

BCH210H © Lisa | Page 12 Lecture 29: Krebs Cycle  phosphatase is continuously eating away at the phosphorylated form and returning it back  calcium will increase activity of phosphatase—return to active form, resulting in ATP production  calcium is released during muscle contraction  regulation of PDH complex  NADH and AcCoA will activate the kinase which will turn off the complex by covalent modification  NAD and pyruvate turn off kinase, therefore sustain enzyme in its active form  PDH will regulate a lot of oxidation in mitochondria, strip off CO , pr2duce acetyl group that will enter Krebs cycle  need a lot of ATP  muscle efficiency depends on how much ATP she can make and how efficient she can make from glycolysis to krebs  needs most effective coordination of 3 processes  tricarboxylic—3 carboxyls in one molecule  for 2 C added, 2 CO are produced (not the same 2 C, just C balance) 2  3 places where NADH is made  NADH are high NRG electrons of reduction which go into ETC and make a lot of ATP  made by dehydrogenases  1 FADH (Q2 ) m2de from the coenzyme FAD  1 GDP  CH from AcCoA is added to ketone group in oxaloacetate through a condensation rxn to make 3 citrate  intermediate needs to be formed and water is needed to cleave thiester form this 6C intermediate  CoA released from water  2C + 4C → 6C  breaking thioester off creates a lot of NRG  favourable rxn  water is needed  C-3 is not chiral  need to relocate –OH on C-3 for future rxs  isomerisation by aconitate  relocates –OH ICDH  first site of NADH production  oxidoreductase rx BCH210H © Lisa| Page 212  reduction of NAD to NADH  part of the dehydrogenase  oxidation  -OH is changed into a ketone group  more of the NRG released is coming from a decarboxylation  moving of –OH allows decarboxylation to occur  oxidation allows more NRG release  6C → 5C  NRG is used to make NADH  very favourable  NADH goes to ETC to be used to sustain lots of ATP generation  source of NRGetic electrons  CO is the second CO released 2 2  first one from PDH complex  (3C from pyruvate, net 3CO pr2duced)  sometimes called oxidative decarboxylation  C=O release (decarboxylation)  -OH to ketone (oxidation) αKGDH  oxidoreduction  release of NADH (reduction)  oxidative decarboxylation  C=O released in CO (t2ird CO rel2ased)  ketone converted to thioester o thioester linkage is high in NRG  favourable
More Less

Related notes for BCH210H1

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.