BIO130H1 Study Guide - Dna Ligase, Okazaki Fragments, Dna Replication

51 views3 pages
1 Apr 2013
School
Department
Course
Professor
ash-mr18 and 40043 others unlocked
BIO130H1 Full Course Notes
31
BIO130H1 Full Course Notes
Verified Note
31 documents

Document Summary

Cell wall: protection and flagella & cilia (some) Semiconservative: each new daughter dna contains 1 strand of parental dna. Synthesized from 5"-3"; template is read from 3"-5". Ars can be inserted into plasmids and can fxn as an origin of replication (histidine gene experiment on yeast) Primers: unidirectional growth, 2 origins (linear virus, unidirectional growth, 1 origin (some plasmids, bidirectional, 1 origin (eukaryotes and bacteria) Multiple origins, many are usable but a few are most commonly used. Single origin (cicular), bidirectional growth, 2 replication forks: initiator proteins bind to origin, loading protein helps helicase bind (atp needed), Naps(nucleoid-associated proteins): h-ns, histone-like proteins: hu, ihf, fis. Genome is packaged into chromosomes (chromosome painting hybridization, incorporating a fluorescent label, view in karyotype to see array of paired chromosomes) Humans: 23 pairs, single long linear dna molecule + proteins. Tightly packaged, but accessible for transcription, replication and repair, dynamic.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers

Related textbook solutions

Related Documents

Related Questions