BIO130H1 Study Guide - Final Guide: Nucleosome, Polyacrylamide Gel Electrophoresis, Agarose Gel Electrophoresis
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BIO130H1 Full Course Notes
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Bio130 lab 1: dna isolation and agarose gel electrophoresis. Basic building block of dna: nucleotide consisting of 5c sugar deoxyribose to which 1 phosphate esterified at. 5" position of sugar ring, 1 nitrogenous base attached at 1" site. Nucleoside: molecule consisting of 1 nitrogenous base lined to pentose sugar moiety (deoxyribose sugar: nucleoside = deoxyribonucleoside) Covalently linked to form linear polymer, backbone composed of alternating sugar and phosphate groups joined by 3"-5-phosphodiester bond. Base composition: ratios of 4 component bases variable from one type of organism to another, constant for species (#purines = #pyrimidines) Dna structure: 2 chains of nucleotides, chains spiral around each other to form pair of right handed helices, chains run in opposite directions (antiparallel) One chain aligned in 5" 3" direction, partner aligned in 3" 5" direction (polarized) Waals forces provide stability (0. 34nm between each base: strands held together by h bonds between associated bases on each strand.