BIO230H1 Study Guide - Final Guide: Nuclease Protection Assay, Ribosomal Rna, Sedimentation Coefficient
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BIO230H1 Full Course Notes
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Bio230 lab 3 study notes: rapid isolation and electrophoresis of rna from e. coli. 3 main classes of rna in bacterial cells: ribosomal rna (rrna, transfer rna (trna, messenger rna (mrna) Most abundant + stable in most cells = rrna. Separate it according to size via sedimentation or gel electrophoresis. Most rna detectable in gel is either rrna or trna. Primary transcripts of prokaryotic rrna molecules about 30s in size. Contain one copy each of 16s, 23s, 5s + trna precursors (v. small) Each time operon is transcribed get 1 copy of each rrna needed for ribosome. Multiple copies of the rna operon on the genome. Ex: e. coli genome has 7 copies of rna operon. Measures rate at which particles move in the gravitational field in ultracentrifuge. Sedimentation coefficient = rate the solute suspended in a less dense solvent fall. = rate per unit centrifugal field as a function of weight and shape.
