BIO230H1 Study Guide - Final Guide: Live Cell Imaging, Fluorescence Microscope, Cytoplasmic Streaming
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BIO230H1 Full Course Notes
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Bio230 lab 4 study notes: live cell imaging. Resolution (ability to distinguish between 2 closely positioned objects) Resolution = minimum distance by which 2 objects can be separated and still distinguished as separate objects. Shape of the cone of light entering objective lens (numerical aperture) Can magnify cells up to 1000 times. Resolve details as small as 200 nm. Limit to resolving power due to wavelength of visible light (not. 3 requirements to view cells: bright light focused onto specimen by lenses in condenser, specimen has to allow light to pass through, appropriate set of lenses arranged to focus image quality of lenses) Have compound microscope used in conventional bright field light microscopy. Compound b/c has several lenses that magnify and focus light (objective, ocular, condenser) Align to get max lighting + resolution (koehler) Gives contrast in wet mounts, unstained and unmounted specimens. Use 2 light filters (only indirect refracted and diffracted light goes to specimen.
