CSB328H1 Study Guide - Final Guide: Lac Operon, Coevolution, Lac Repressor
25 Jan 2014
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At rich in e coli and salmonella first genocmic experiment (analysis of bacteria by chargaff. 1. this gives the cell the ability to take in dna but buffers any negative fitness consequences if you get rid of hns, the at rich will increase dramatically. 32. incubate the dna and then fluorescently label dna and put it on microarray slide and the fluorescing genes correspond to the genes that were boundto h-ns. 33. if you now look at the salmonella genome, plotted for its gc content, every spikeis a spike in the. At rich dna has diff properties than gc rich dna: 1. Dna becomes more flexible: minor groove of at rich dna is deeper bc, gc rich dna has an extra cyclic aminogroup poking out, solved the sturctre of hns, dna biding domain has conserved motif. 23: at hook motiffits snuggly into the at rich minor grove of dna.
