CSB428H1 : Article #2 Summary
Document Summary
os discs are renewed whereby rhodopsin-laden vesicles in the os axonemal cytoplasm fuse with nascent discs abundant in pi3p and fyve domain protein sara interacts with pi3p, rhodopsin and syntaxin 3 snare to regulate this process. rhodopsin"s c" tail has an address signal to os and also binds sara. Fyve domain is a conserved double zinc finger motif that binds pi3p specifically. Sara (smad anchor for receptor activation) has fyve and is located on ee. In yeast two hybrid and gst pull down assay, rhodopsin c" interacts with human sara. Ab for rhodopsin also co-immuniprecipitated endogenous mouse sara. In hek cells, sara colocalized with eea1 and rhodopsin on ee. In rods, sara is found in punctate ee in apical cytoplasm of is and basal portion of proximal axoneme including the connecting cilium and basal body. Sara-pi3p interaction is necessary for rhodopsin delivery to os.