CSB428H1 : Article #4 Summary

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crimsonwombat705

24 Dec 2010

School

UTSG

Department

Cell and Systems Biology

Course

CSB428H1

Professor

U.Tepass

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Document Summary

Pten localizes to apical pm to increase pi45p2 by decreasing levels of pi345p3. Ectopic pi45p2 at basolateral surface cause apical proteins to relocate to basolateral side. annexin 2 (anx2) binds pi45p2 and is recruited to apical surface to bind cdc42, which recruits apkc. Loss of pten, anx2, cdc42 or apkc prevents apical surface and lumen formation. madin-darby-canine kidney (mdck) cells embedded in gels for cysts with lumen. Ph domain of plc fused to gfp (phd-gfp) detects pi45p2. Phd-gfp localized mainly to ap pm and little bit in bl pm, similar to that of actin. Ph-akt-gfp detects both pi34p2 and pi345p2 which are enriched at bl pm. before lumen formation, pi345p3 and pi45p2 localized to cell-cell and cell-ecm contacts. During lumen formation, pi45p2 became confined to ap pm, but pi345p3 became exclusive to bl. In early stages, gfp-pten is localized to the cytoplasm but later is localized to ap pm and overlapped with pi45p2 and cell-cell junctions.

Related Questions

TRUE OR FALSE FOR 31-36

31. GLUTs are uniporters that use ATP hydrolysis for energy.

32. GLUT1 transports glucose across the blood brain barrier.

33. GLUT2 initially transports glucose into the pancreas after eating, allowing insulin to be released into the bloodstream.

34. After insulin signaling, GLUT4 is transported to the membrane of various cells to increase glucose transport in tissues (But not if you have Type II Diabetes).

35. If the plasma membrane of an animal cell was made entirely permeable to Na+, the Na+/K+pump would continue to pump ions and hydrolyse ATP, but not build a Na+gradient.

36. Passive carriers are localized to the basolateral membrane of epithelial cells, activate transporters are localized to the apical membrane.

You are interested in epithelial tissue formation and work in a lab studying MadinâDarby canine kidney (MDCK) cells. MDCK cells spontaneously form hollow epithelial vesicles. Staining these vesicles with an anti-actin antibody demonstrates the existence of apical microvilli near the central cavity of the vesicle. A new postdoctoral fellow in the lab comes to you in a panic. You had given her some dissociated MDCK cells that have been cultured as single cells in a collagen matrix. Because the cells did not look obviously polarized to her, she stained the dissociated MDCK cells to visualize the microvilli and she does not see any signs of polarized microvilli localization in these cells. Explain why you are confident that you did not give her the wrong cell line

A. Her cells did not look polarized because she used an anti-actin antibody to reveal microvilli, rather than an anti-tubulin antibody. B. Her cells failed to polarize because she grew them in a collagen matrix instead of laminin. C. Her cells failed to polarize because she grew them dissociated instead of in a collagen matrix. D. Her cells did not show microvilli because MDCK cells only form epithelial vesicles in the presence of epidermal growth factor (EGF) which she did not add to the culture medium.

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1. Which mutation is an example of an adaptation?â

	a.	A mutation results in decreased sperm count in humans.â
	b.	âA mutation is found to be the cause of Alzheimer's Disease.
	c.	âA mutation results in hairless cats, reducing allergies in humans.
	d.	âA mutation increases the size of tomato plants.
	e.	âA mutation renders an individual immune to HIV infection.

2. The prokaryotic chromosome is comprised of a ____.

	a.	âhighly folded mass of a single, linear molecule of DNA
	b.	âloosely folded mass of a single, linear molecule of DNA
	c.	âhighly folded mass of a double, circular molecule of DNA
	d.	âhighly folded mass of a single, circular molecule of DNA
	e.	âloosely folded mass of a single, circular molecule of DNA

3. Which methodology was used by researchers to identify the nuclear localization sequence?â

	a.	infection of cells in culture with viruses containing sequential deletions of amino acids in a protein known to localize to the nucleus, followed by microscopic determination of cellular localizationâ
	b.	âtreatment of cells in culture with pharmacological agents that inhibit the nuclear pore complex, followed by microscopic determination of cellular localization
	c.	âtransfection of cells in culture with mutant human proteins containing sequences believed to be required for nuclear localization followed by microscopic determination of cellular localization
	d.	âfluorescently labeling cells with known nuclear proteins and tracking changes in localization in the presence or absence of nuclear pore complex proteins
	e.	âtreatment of cells in culture with pharmacological agents that binds to the nuclear localization sequence and prevents nuclear transport, followed by microscopic determination of cellular localization27. Cells actively secreting enzymes would likely carry out more ____ than other cells.â

4. Cells actively secreting enzymes would likely carry out more ____ than other cells.â

	a.	exocytosisâ
	b.	âosmosis
	c.	âendocytosis
	d.	âconjugation
	e.	âFractionation

5. What is a function of prokaryotic common pili?â

	a.	âbiofilm formation
	b.	âtransfer of DNA
	c.	âprotein polymerization
	d.	âenergy production
	e.	âlipid synthesis

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CSB428H1 : Article #4 Summary

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