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CSB428H1 Final: 2009 EXAM ANSWER

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crimsonwombat705
24 Dec 2010
School
UTSG
Department
Cell and Systems Biology
Course
CSB428H1
Professor
U.Tepass

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Document Summary

Drosophila sec5, sec6 and sec15 tethers de-cad vesicles originating from the re to lateral membrane for exocytosis. Sec10 at pm and arm act as a landmark for de-cad delivery. In the absence of sec5, sec6 and sec15 function, de-cad accumulates in an enlarged re. Key experiment sec5 mutant notum cells generated using flp/frt were analyzed for different intracellular markers to investigate the nature of the enlarged intracellular compartment. Golgi marker lava lamp, late endosome marker hepatocyte growth factor-regulated tyrosine kinase substrate, early endosome marker rab5 was distributed normally in mutant cells. But rab11 distribution was disrupted in mutant cells: they were found in large punctate structures with higher intensity of rab11 and colocalized with. Additional re maker nuclear fallout confirmed this result. This shows sec5 is necessary for the recycling of de-cad from re to the lateral membrane. Investigate possible interaction between components of the exocyst complex with the cell cytoskeleton.

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Related Questions

Write true or false and please explain

30. In secretory cells, the constitutive and regulatory pathways ofexocytosis diverge at the cis Golgi network. ------------------ ( )( )
31. Many proteins are posttranslationally glycosylated at tyrosineresidues in the ER.------------------------------------------------- ( ) ( )
32. Cells import LDL molecules exclusively through pinocytosis.-------------------------------------------------------------------------( ) ( )
33. Cells recycle LDL receptors from lysosome to the plasmamembrane.----------------------------------------------------------------- () ( )
34. Phagosomes fuse with late endosome.------------------------------------------------------------------------------------------------------( ) ( )
36. If a Sec mutant showed an accumulation of newly synthesizedproteins at ER,
then the Sec gene is likely a component of vesicle-buddingmachinery of trans-Golgi network.------------------------------------ ( ) ( )
37. A signal recognition particle is a single pass trans-membraneprotein of the ER membrane.---------------------------------------- ( ) ( )

d. The exact intracellular trafficking pathways that affect delivery are also unclear. A recent report showed that for a leading lipid delivery system, ~70% of the siRNA taken up by cells underwent endocytic recycling and exocytosis109. An improved understanding of both endosomal release and intracellular trafficking may facilitate the design of delivery systems.

what is this mean?

what is t intracellular trafficking pathways?

Which compartments are involved in vesicular transport? How are transport vesicles formed and targeted appropriately? What proteins are involved and what are their roles? What kinds of modifications are added to proteins in the ER? What determines whether a protein stays in the ER or moves on? Be sure to understand how vesicles fuse with their target membranes, and the consequences of fusion for the vesicle’s cargo as well as the proteins and lipids that are in the membrane. Know the structure of the Golgi apparatus and its role in intracellular trafficking. Understand the secretory process, endocytosis, exocytosis, function of the endosomes, and function of the lysosomes. Understand why endocytosis happens and how it can be regulated to take up specific molecules.