CSB452H1 : Summary of Lecture 1

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Pathogen associated molecular pattern can be flagellin, lipopolysaccharides of gram-negative bacteria, chitin, ergosterol, transglutaminase from fungi, or bacterial translation elongation factor ef-tu. Induces mapk cascade, transcription of pr genes and deposition of b1-3-glucn callose at infection site visualized by analine blue dye. Pamp cause plant to uptake h+, release k+ and increase ph causing alkalinization of media. apply bacterial protein extract separated by sds-page and see which band causes alkalinization. when flg22 is applied to plant before infiltration, bacterial growth decreased afterwards. Plants are then selfed to produce homozygous recessive mutant. wt seedling exposed to flg22 show stunted growth, so screen for insensitive mutant that grows normally with flg22. They do no accumulate callose and are more susceptible to. Pseudomonas syringae infection, growth and invasion into tissue using spray method. Infiltration method caused no difference showing fls2 is not involved in post invasion defence.

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