1. Genomic DNA is digested with a restriction enzyme that has a 4 bp recognition (restriction) site. What would be the expected average size of the fragments that would be produced by digesting DNA of random sequence with this enzyme? A) 8 bp B) 16 bp C) 125 bp D) 256 bp E) 512 bp
2.insertion or removal of one or more nucleotide base pairs in DNA within a gene often results in a ____________ mutation. A) transition B) frameshift C) reversion D) transversion E) suppressor
3. Which ingredient is NOT required in Sanger DNA sequencing? A) Primers for DNA polymerase. B) Reverse transcriptase. C) Dideoxy nucleotides. D) Deoxy nucleotides. E) DNA template.
4. What is the purpose of Taq polymerase in a PCR reaction? a. DNA denaturation b. Primer annealing c. DNA synthesis d. Heating of the reaction e. All of the above are associated with Taq polymerase.
5.You are doing lab work with a new species of beetle. You have isolated lines that breed true for either blue shells and long antenna, or green shells and short antenna. Crossing these lines yields F1 progeny with blue shells and long antenna. Crossing F1 progeny with beetles that have green shells and short antenna yield the following progeny: Blue shell, long antenna 92 Green shell, short antenna 88 Blue shell, short antenna 31 Green shell, long antenna 29 Total 240 Assuming that the genes are linked, what is the map distance between them in cM? A) 75.0 cM B) 25.0 cM C) 12.1 cM D) 12.9 cM E) The genes are actually assorting independently. Genes A and B are linked with 10 map units (cM) between them. A heterozygous individual AB/ab (note that the dominants are in coupling) would be expected to produce gametes in which of the following frequencies?
A) 45% AA; 10% Ab; 45% bb
B) 5% AB; 45% Ab; 45% aB; 5% ab
C) 10% AB; 40% Ab; 40% aB; 10% ab
D) 45% AB; 10% Ab; 10% aB; 45% ab
E) 45% AB; 5% Ab; 5% aB; 45% ab