HMB265H1 Study Guide - Final Guide: Chromosome, Model Organism, Brca1

• School: UTSG
• Department: Human Biology
• Course: HMB265H1
• Professor: Maria Papaconstantinou

Document Summary

Hartwell et al. n/a: gain-of-function alleles are usually, more severe than null, conditional, amorphic, haplosufficient. Qtl analysis was performed to identify the genomic region associated with that trait. Near-isogenic lines (nils) were used to narrow down the qtl region. The molecular analysis of the recombination breakpoints of the nils is shown in the figure below. The solid or dashed lines represent the dna of the two parental lines used in generating the nils. Which of the 4 genes represent factor x: 1, 2, 3, 4. Approaches to gene cloning: positional cloning identifying the gene based on its position and based on its phenotype. If we have two loci that are closely linked together, the chances that we have recombination events occurring between them is very low (lower the closer the loci are) If we can track recombination events, we can figure out how close or how far the two loci are from each other.