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IMM250H1 Study Guide - Mhc Class Ii, Mhc Class I, T-Cell Receptor


Department
Immunology
Course Code
IMM250H1
Professor
Julius

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Some in intestinal epitheliums but restricted there
T cells mature in Thymus
B cells mature in Bone Marrow
Intestinal Intra Epithelial Lymphocytes (iLEL) developed T cells have no access to rest of organism
Despite reduced T cell production as you grow
Lymphocyte homeostasis: Blood and secondary lymphoid organs have roughly constant number of T and B cells
Igb with H-2b
Allotype specific antibody (against an antibody) will not recognize antibody of a different allotype
After F1 backcross 50% pups heterozygous for selected allotype
Further crossed to create 1:3 homozygous
Congenic Strain
n= number of crosses
1-(1/2)n
Ab (Ig) produced by B cells
High does of Ag activates high and low affinity Ab B cells
Low doses of Ag activates only high affinity Ab B cells
Ab produced in initial response has lower affinity than those produced later
Frequency for B cell for any Ab is 10-5
Affinity to be considered Ab: ~105
Ag concentration determines affinity of Ab
Freund's Adjuvent: Mineral oil containing Ag
Somatic mutation: After B cell maturation
T cell activation requires APC with compatible MHC
CD4/CD8 denotes restriction- not function
CD8
MHC I
CD4
MHC II
MHC alleles co-dominant
Incubate with APC of certain MHC allotype and Ag
Isolate adhered and not adhered
Pan experiment
MHC Loci
Mouse Strain
K
I-A
I-E
D
Class
I
II
II
I
BALB/c (H-2d)
d/d
d/d
d/d
d/d
C57BL/6 (H-2b)
b/b
b/b
Chain defect
b/b
F1 (H-2d/b)
d/b
d/b
d/-
d/b
T cells react to MHC of the species (allogeneic) but not to self MHC
1% of T cells in population recognize allogeneic MHC
Self MHC on TE
MHC expressed on thymic epithelium (TE) positively and negatively selects T cell repertoire
Appropriate MHC in APC still needed for activation
Precursors for any H-2+Ag T cell exist- but must be positively selected
MHC on TE only positively selects
MHC of stem cells used to reconstitute host will be MHC expressed by T cells, B cells and APCs
MHC of intra-thymic stem cells and developing T cells will negatively select self-reactivity
MHC on TE negatively select T cell TcR which are not deleted by donor stem cells
MHC on TE positively select self-MHC+Ag
MHC expressed by APC determines activation
Chimera Rules
Julius' Basic Rules
IMM334 Page 1
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