MGY277H1 Final: MGY277 - Final Exam Notes

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Unit 1 the scope of the problem. Lister: pioneered antiseptic surgery, sterilized wounds and surgical instruments with carbolic acid (phenol, phenolic compounds kill lots of bacteria but are non-toxic enough to be added to personal care products. Directs light towards specimen at an angle, only light scattered by specimen enters objective. Cheaper, simpler can see up to visible spectrum (400-750 nm) Electrons scanned over surface, released electrons are reflected and observed. Cells stand out bright against dark background (cid:894)thi(cid:374)k a(cid:271)out ho(cid:449) lase(cid:396) (cid:272)a(cid:374)"t (cid:271)e see(cid:374) f(cid:396)o(cid:373) side u(cid:374)less the(cid:396)e"s s(cid:373)oke(cid:895) Wavelength 1000x shorter than light, 1000x more resolution (0. 3 nm) Must be in vacuum, images in black and white, see more details, cell must be dead. Thin sectioning can see internal details, but can distort cells: gram-stain (stains inside of cell, not cell wall, crystal violet stain (primary stain) cells are purple.

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