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MGY299Y1 : Lab notes

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redtermite204
19 Dec 2011
School
UTSG
Department
Molecular Genetics and Microbiology
Course
MGY299Y1
Professor
Johanna Rommens

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Document Summary

Conjugation steps: physical contact, mobilization/rolling circle replication, transfer, 1 phenotype can be caused by multiple genetic regions. Strain 1 has a mutated; strain 2 has b mutated both no growth. Make third strain with genetic mutations of both if growth => complement, different genes if no growth => same genes. Can also have recombination or reversion to get wild-type both these are rare. If get lysis (wild-type), complement but in different complementation groups. Do not get complementation, not in same complementation groups, same genes. Control is spotting the phage onto the same phage should not complement plaque in such cases must be reversion, because cannot recombine. Essential gene identification essential genes refer to conditional mutants: grow at permissive temperature, to suspect if gene is essential fwd and rev part of plasmid, not genome.

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Related Questions

Determine which is the result of complementation or which fails to complement in terms of gene interaction. The two options for answers are as follows: a) complementation or b) not complement Please spell the answers and report in all lowercase correctly to receive full points. Thus, the answer to the question shall either be "complementation" or "not complement".

Questions for determination:

1) Wild type progeny Blank 1

2) Not wild type progeny Blank 2

3) Null mutation Blank 3

4) Recessive mutation Blank 4

5) Alleles of the same gene Blank 5

6) Alleles of different genes Blank 6

Wildtype harebells have blue flowers. Two mutant strains of harebells have recessive, white flowers. First, describe in detail an experiment to test whether the mutations in these two strains of harebells affect the same gene. Second, describe in detail an experiment to determine how the two genes interact if the mutations happen to affect different genes. Underline the following terms in your answer: complementation, dihybrid cross, haplosufficient, and phenotype ratio.

Wildtype harebells have blue flowers. Two mutant strains of harebells have recessive, white flowers. First, describe in detail an experiment to test whether the mutations in these two strains of harebells affect the same gene. Second, describe in detail an experiment to determine how the two genes interact if the mutations happen to affect different genes. Underline the following terms in your answer: complementation, dihybrid cross, haplosufficient, and phenotype ratio.