MGY377H1 Midterm: Study notes for midterm

63 views3 pages

redtermite204

25 Dec 2011

School

UTSG

Department

Molecular Genetics and Microbiology

Course

MGY377H1

Professor

William Navarre

For unlimited access to Study Guides, a Grade+ subscription is required.

Document Summary

Central dogma = dna into rna into protein that carries out particular function. Gene = stretch of dna in a genome that encodes a discrete and individual protein or rna. Operon is cluster of genes under same regulatory control. Monocistronic mrna is a single rna that codes for single protein. Polycistronic mrna is single rna that codes for multiple proteins. Regulons are operons around chromosome that share regulation. Regions of dna that control transcription of adjacent genes are called promoters. Promoter is region of dna that binds dna polymerase to initiate transcription of an adjacent gene. A -35 hexamer centered approximately thirty five nucleotides upstream of transcription. Up element rich in at base pairs. Consensus sequence of typical promoter determinants of promoter strength are sequences of the negative ten and negative thirty-five boxes and the amount of space between them: good promoter is reca. closest to census: bad promoter is arabad.

Related Questions

6. Proteus mirabilis is a bacteria that causes urinary tract infections. Its Ure operon (genes UreA-G) allows it to metabolize urea present in urine. Transcription at the Ure operonâs promoter is controlled by the UreR protein. Transcription of the UreA-G genes is on when urea is present and off when urea is absent. The UreR protein binds to urea and this urea binding is required for UreR to bind DNA. In response to urea, what kind of regulator is this? Explain how you know

brownfox792

Answer the following True or False questions and briefly explain your answers.

True or False EXPLAIN: Most human cells, in a single individual, contain the same amount and type of DNA

True or False EXPLAIN: Most human cells, in a single individual, contain the same amount and type of protein.

True or False EXPLAIN: Once a gene is transcribed, it is always translated immediately.

True or False EXPLAIN: RNA polymerase binds to the promoters of all genes at all times, so that it is prepared for transcription.

True or False EXPLAIN: Some transcription factors are general (they play a role in the transcription of most or all genes), while other transcription factors are specific to just a few genes.

True or False: EXPLAIN Turning genes on at the right time and place requires both specific regulatory DNA sequences and specific regulatory proteins.

1.For each of the following items, which refer to bacterial genes, indicate whether it is / or functions at the level of, DNA, RNA, or protein:

a.RNA polymerase sigma factor (DNA, RNA or protein)

b.Promoter (DNA, RNA, protein)

c.-10 TATA box (DNA, RNA or protein)

d.transcription termination signal (DNA, RNA or protein)

e.operator (DNA, RNA or protein)

f. repressor (DNA, RNA or protein)

silverturtle620

MGY377H1 Midterm: Study notes for midterm

Document Summary

Get access

Related Documents

MGY377H1 Lecture Notes - Lecture 13: Regulon, Consensus Sequence, Operon

MGY377H1 Lecture Notes - Lecture 16: Alphaproteobacteria, Cistron, Enzyme

BIOB11H3 Lecture Notes - Lecture 8: Response Element, Histone Deacetylase, Leucine

Related Questions