MGY377H1 Midterm: Study notes for midterm

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redtermite204

26 Dec 2011

School

UTSG

Department

Molecular Genetics and Microbiology

Course

MGY377H1

Professor

William Navarre

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Document Summary

Important for how cell organizes cell, both eukaryotic and prokaryotic. Lipid bilayer highly impermeable to anything big, glucose, anything bigger than glucose, proteins are synthesized inside cell. Proteins must leave cell like cholera toxin, a protein, All require energy which are from different sources. Chaperone proteins most: bind to cargo proteins do not let fold up into globular shape, keep into secretion competent linear shape. Homologues apparatus that pumps proteins out of cell have phage tail and have homology to. Flagella also look like type 3 secretion systems evolved for one purpose, then adopted for another purpose in evolution. Protein to be secreted carries some sort of targeting signal: that the secretion system will recognize, n-terminal sequence. Sec system allows for proteins to be secreted across cytoplasmic membrane. Different pathways for proteins that go across and proteins that into membrane. For a protein to cross membrane, occurs post-translation protein almost completely syntehsized.

Related Questions

1. The histones that form the core of the nucleosome get into the nucleus by which of the following?

a) Passive diffusion through nuclear pores

b) Selective and facilitated active transport through nuclear pores

c) Ribosomes located in the nucleus synthetize histones in the nucleus

d) Histones are made at the ER membrane and then diffuse to the nucleus

2. Membrane proteins that belong to the golgi, lysosome, or plasma membrane first pass through which organelle before reaching their final subcellular destination?

a) The golgi apparatus

b) The smooth ER

c) The nucleus

d) The ER membrane

e) The ER lumen

3. Where in the cell would a ribosomal protein be located if it has had its nuclear export signal inactivated (non-functional or non-existent).

a) The protein would be found in the ER lumen

b) The protein would remain in the nucleus

c) The protein would be secreted

d) The protein would remain in the cytoplasm

e) The protein would be in the ER membrane

4. Where would be the final destination of a protein if it contained a NLS anywhere in the protein sequence (and which is exposed to the cytoplasm after protein folding) but also contained an ER signal sequence at the carboxy terminus?

a) The ER lumen

b) The nucleus

c) The cytoplasm

d) The inner nuclear membrane

e) The ER membrane

5. The sequence K-D-E-L serves as a retention/retrieval signal for ER proteins.

a) True

b) False

6. The following are true for both cell membrane proteins and secretory proteins EXCEPT?

a) Precursors of the mature proteins contain signal sequence.

b) Both types of proteins contain stop-transfer sequences

c) Both types of proteins enter the ER while being synthetized

d) Both types of proteins pas through the golgi apparatus

e) Both are carried by clatherin-coated vesicles

7. If you isolate a regulated secreted protein from a secretory/storage vesicle and inject it into the cytosol, is will?

a) Be secreted through channels in the plasma membrane

b) Be taken up into secretory vesicle and secreted

c) Remain in the cytosol until it is degraded

d) Be taken up into the rough ER and follow the secretory pathway

goldmanatee250

Will proteins that are secreted by the Sec translocase during growth in laboratory media be secreted by the Tat translocase during infection?

Do the sec translocase contain proteins in the cytoplasm and the inner membrane?

In a eukaryotic cell, proteins are synthesized, and pass through a series of specialized organelles before reaching their target. Describe how a trans-membrane protein would be synthesized, processed and ultimately become a part of the plasma membrane of the cell.

MGY377H1 Midterm: Study notes for midterm

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