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Department
Molecular Genetics and Microbiology
Course
MGY377H1
Professor
William Navarre
Semester
Fall

Description
05 Purification of antibodies In gram positive no outer membrane thick peptidoglycan layer Sorting signal of grampositive surface proteins LPXTG is the sorting sequence Protein A is made on a ribosome in the CYTOPLASMProtein temporarily anchored to membrane because charged and hydrophobic domains Sortase enzyme Peptide bond attaches protein A to peptidoglycan newly built subunitErgo protein A is anchored to peptide subunit of peptidoglycano Via transpeptidation and transglycoslyation reactionso Incorporate protein A into existing peptidoglycan chain Teichoic acid and lipotechoic acid are major components of gram positive cell wall TAFound only in gram positive bacteriaGram negative bacteria have only outer membrane Attached to NAM one of two sugars in peptidoglycanon carbon six of NAM NEGATIVE chargephosphate group Function unknown
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