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Department
Molecular Genetics and Microbiology
Course
MGY377H1
Professor
William Navarre
Semester
Fall

Description
25Conjugation PlasmidsSmall circular supercoiled DNA molecules Present in most species but not all strains May have one to thousand copies per cell Use replication machinery of the hosto May require proteinencoded proteins Have own replication originAre dispensable for growth under most conditions But there are exceptions to each of these rules Stably maintain themselves in the cell through common strategies o Autonomous replication and copy number controlo Providing useful function for the cell such as a drug resistance o Partition systems that actively segregate the plasmid to daughter cello Addiction systems Plasmid replication Origin of replicationo May be unidirectional or bidirectionalo When bidirectional replication forks may move differently Replication proteinso Requirements varygenerally use host chromosomeencoded with or without pl
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