PCL477H1 Study Guide - Final Guide: Embryonic Stem Cell, Hematopoietic Stem Cell Transplantation, Tunel Assay

• School: UTSG
• Department: Pharmacology and Toxicology
• Course: PCL477H1
• Professor: J.Peter Mc Pherson

Document Summary

Dna damage response in vivo: cell lineage effects. Cell lineage, very simple but profound, irradiate mice with varying doses: mice die of different things happening depending on the dose, 12-13 gy they die due to bone marrow annihilation (aplasia), If mice are given bone marrow transplant they live: 15 gy the mice die a lot faster 6-8 days by gi tract damage. Cannot be rescued by bone marrow transplant. Postulated to target epithelial stem cells to initiate lethal gi syndrome. Above 8gy dose-dependent stem cell death leads to dec. crypt regeneration. Dna damage response in vivo: cell lineage effects and development * Microvasular endothelial apoptosis after 15gy: faster onset of apoptosis in lamina propria. There exists a threshold for massive endothelial apoptosis. Different lineages got affected at different rates. Endothelial cells in capillary die first and contribute to more death. Example 2 dna damage and cns development *