1) The following equilibrium constants have been determined at 298K for these two reactions that are part of the citric acid cycle (TCA or Krebs cycle).
I AcetylCoA + oxaloacetate + H2O -->
<-- CoA + citrate
II L-malate + NADox -->
<-- oxaloacetate + NADred
Given that KI = 6.97 x 107 and KII = 8.87 x 10-6, calculate the standard state transformed Gibbs free energies of reaction for each of these reactions, â rG'o .
I have gotten values of â r IG'o
= -4474 J/mol and â r IIG'o = 28821 J/mol
b. For reaction II, the mitochondrial matrix has the following concentrations of components under steady state condition:
[NADox] =1.0 mM [NADred] = 0.1 mM
[malate] = 3.5 mM [oxaloacetate] = 0.0003 mM
What is the solution potential of the NADred/NADox redox couple?
I am not sure how to proceed with this. I think I need to use the equation E = E0 âRT/abs(v)F ln K
Are these two redox couples at or near equilibrium? Explain your answer.