MICR 302 Study Guide - Midterm Guide: Lac Operon, Regulon, Sigma Factor

• School: University of Victoria
• Department: Microbiology
• Course: MICR 302
• Professor: Briant

Document Summary

Study: puf ng pattern induced by temperature shock and dnp in drosophila. In early drosophila development the dna is replicate but does not separete. Remains attached @ centromeres and copies a lot. This is actually visible under a microscope (powerful tool when studying gene expression) When heated, the poly2 chromosomes underwent puf ng in a location that corresponded to heat shock genes (induced in response to stress, found in all organisms) Names because of this reaction to heat but are actually a general reaction to various types of stress, more commonly known as chaperones . Heat-shock respone > sigma 32 aka sigma h (rpoh > rna polymerase gene) Course of action: heat shock (38 -42 c for e coli, sigma regulon activation (regulon = genes with same regulatory mechanism and separate promoters) 50 different promoters (turned on or off according to its presence or absence: chaperones (groel, dnak) and protease expression (lon, cip, ftsh)