Study Guides (248,419)
Canada (121,550)
55-213 (12)
Final

55-213 lab 2 exam review.docx
Premium

1 Page
94 Views
Unlock Document

Department
Biological Sciences
Course
55-213
Professor
Hubberstey
Semester
Winter

Description
Isolation of Genomic DNA - Genomic DNA- used for DNA structure, DNA hybridization, gentic studies and DNA-protein interactions - Procedures: PCR, sequencing or cloning - Best way to extract DNA (gold standard): Marmur method o 2 barriers: cell membrane and cell wall o Cell is disrupted from bacterial cell wall by physical or chemical methods (detergents and hydrolytic enzymes such as lysozymes)  Gram negative cells (like E. Coli) only require detergent o Chlorofoam is used to remove protein components from the cell lysate Flow chart - Oakridge rube with E. coli in saline-EDTA cells are in the logarithmic or early stationary phase of growth o NaCl- o Saline EDTA- metal ion keylator First, it binds divalent metal ions (Cd", Mg 2 +, Mn 2 +) that could form salts with the anionic phosphate groups of the DNA. Second, it i
More Less

Related notes for 55-213

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit