[Biology 2382B] - Midterm Exam Guide - Comprehensive Notes for the exam (42 pages long!)
6 Feb 2017
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Growing isolated, broken down tissue in an artificial environment. Broken down: cells have to be removed from the extracellular matrix (sticky proteins) by enzymes (trypsin) and removing calcium (proteins need calcium to function) Amino acids, minerals, vitamins, salt, glucose, etc. Contains growth factors from blood, help stimulate cells to divide insulin , for sugar transport. Must be placed in a incubator (mimic body conditions) Cells grown in suspension require mechanical action to prevents cells from sinking down. Primary cell culture are taken directly from the organism. Have contact inhibition (stop dividing when they touch each other) Can add trypsin to cleave sticky protein to take them out and place them in a new culture (passaging cells) Passaging cells is limited by 50 generations. Won"t divide after, adapt strange morphology (senescence) Can be passaged indefinitely and do not suffer from the hayflick limit or contact inhibition. Introducing genes that override normal cell cycle growth signals.
