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Cell Bio Lecture 1.docx

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Department
Biology
Course
Biology 2382B
Professor
Robert Cumming
Semester
Fall

Description
Stem Cells Primary cell culture Isolating the Cells -Embryonic stem (ES) cells are pluripoten -tells taken directly from an organism -Break cell to cell, and cell to matric -grown indefinitely in culture -finite divisions (~50 generations, Hayflick interactions limit) Growing the Cellsragmentation, trypsin, EDTA Zygote -Contact inhibition if cell density is high -Supplied with proper nutrients; amino acids, -Ultimate totipotent stem cell; capabilityCell line minerals, vitamins, salts, glucose, serum etc. generate every cell type -transformed cells able to grow indefinitely Can grow as: -During Embryogenesis; undergoes symm -HetLa; first human cell line from cervical 1 Adherent Cell Cultures and asymmetric cell division carcinoma biopsy of Henrietta Lacks. -Require surface to grow -Divide finite number of times; cell senescence Asymmetric Cell Division 2 Suspension Cell Cultures -mother cell becom
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