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Biology 2382B Study Guide - Midterm Guide: Cystic Fibrosis, Copii, Protein Targeting

Course Code
BIOL 2382B
Sashko Damjanovski
Study Guide

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Where do Proteins go after the ER?
Proteins go to the golgi from the ER.
From ER to golgi and golgi back to the ER
Lots of vesicles that move between different membranes
Not fully understood how a golgi works
Series of flattened, disk-like, cisternae with no ribosomes
Vesicles at cisterna tips fuse or pinch off
Three types of cisternae (cis, medial, and trans): CGN faces the RER, TGN is opposite to RER,
medial is in the middle
Membranous vesicles moving from the ER to the golgi and then from different parts of
the golgi to endosomes and lysosomes on cell surface.
Processing and sorting of proteins (secreted, membrane, lysosomal)
Transport Vesicles: budding and fusion
Section 5: Vesicular traffic of proteins. Golgi apparatus
Thursday, January 28, 2016
3:52 AM
Midterm 1 Page 1

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Shapes and pullls out the membrane so that they eventually it can form this little vesicle
and pinches the bud off.
Once you've left the ER you need to get rid of the coat proteins
Coat proteins promote budding of vesicles
COPII - anterograde transport - moves from the nucleus towards the outside
COP - retrograde transport - moves from out outside towards the nucleus
They both help to form the bud that pinches off
Coat proteins
V-snares - on the vessicle
T-snares on the target membrane
Snares help with the fusion of proteins
Want them to end up in the golgi
Will bind to membrane cargo receptors
Soluble particle proteins - free floating in the lumen
Membrane cargo proteins - located on the membrane of the ER ( part that stick into the
lumen, transmembrane domain, and a part that stick out into the cytosol)
The part that sticks out into the cytosol interacts with coat proteins
Require help from other proteins - cargo proteins
Membrane associated GTP binding proteins promote association of COPII coat proteins on the
ER membrane.
GTP hydrolyses occurs (turns GTP into DGDP)which causes the release of COPII from the
Exposes the v-snares which can now bind to the t-snares located on the cis face of golgi
membrane of the target membrane.
GTP Binding Proteins Control Assembly and Disassembly of COPII coat proteins
Midterm 1 Page 2

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Diacidic sorting signal (DXE - acidic residues - aspartic X - any amino acid, glutamic acid)
Acidic residues are important for the association of the COPII protein
Cargo membrane proteins are recognized by the COPII coat proteins through
Hydrolysis of ATP promotes the dissociation of the snare complexes (orange bars and
the snares)
We need to break apart the s-snares and t-snares once they have fused and recycle them.
RER to cis-Golgi Transport
COPI proteins will promote vesicle formation from the cis Golgi membrane and form
Retrograde Transport:
Midterm 1 Page 3
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