Biology 3594A Study Guide - Final Guide: Restriction Site, Centromere, Base Pair
7 Dec 2011
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Document Summary
Plap assay - reverse mutation detection assay, not forward. Mutant human sequence was put inside the mouse (g11) and we are looking for the reverse mutation back to g10. The mutation is a single base pair deletion. Cii assay - the mutation target is the cii gene. We sequenced about 400 nucleotides, however only 269 are the effective mutation target. Define mutation target and effective mutation target (where you can detect the mutation). Lesser number of sites where you can actually see the mutation. Tcga - a restriction site in rmc, this was the mutation target for random. The mutation target and the effective mutation target are the same. We are looking for it to be destructed, mutations can eliminate that restriction site. Entire mitochondrial genome was both the mutation target and the effective mutation target in the ultra deep sequencing study. You could extend sequencing to the entire nuclear genome, however it is quite costly.
