Biology 3594A Study Guide - Phenotype, Mutation
Document Summary
Takes the sequence and plots them above the actual sequence. Exception: found that there are mutations that occurred in young harlequin cerebellum and are called class ii deletions. They are intra- chromosomal and they are 10-21 base pairs long. It occurs in the young mice but not in the older seven month old mice. At three months, there is not a lot of neurone loss. At seven months, the mutant cells have died. Intra-chromosomal means within in the same stretch of dna. Only one strand was needed to do this. Plap: detects an increase in mutant frequency, gii is sensitive and unstable, long, more sensitive target, revertant assay - in order to detect mutant frequency, the mutant (g11) needs to come back to g10. Only need a little bit of enzyme to get blue: because of transcriptional error, we already know it doesn"t replicate very well at the dna level, it is also prone to transcriptional error.