Biology 3594A : Ultra Deep Sequencing Notes
22 Dec 2011
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Document Summary
Compact genome, vast majority of it is coding. Heteroplasmy - mixed maternal and paternal inheritance (ultra deep sequencing can find what the probability of inheriting mitochondria from dad. Cytochrome c, rrna, trna, some of the genes related to oxidative phosphorylation. D loop - can have deletions and insertions, base substitutions - can be highly variable but neutral. Theory - aging is driven by mitochondrial disease, mitochondrial function is compromised (oxfos - oxidative phosphorylation, the production of atp. Oxidative damage includes dan, lipids and proteins as an organism ages. High incidence of ros - super oxide anions and hydrogen peroxide are the ones you should remember. Theory - aging is driven by mt disease and mt function is compromised, oxphos and atp production is declined. If this is related to ros, we should see g to t transversions in mtdna and deletions in mtdna. Figure in paper - across the top would be 100% of the genome is sequenced.
