Biology 1001A Final: Final Exam Outcomes

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Lecture 18: optogenetics: basic mechanism of propagation of action potentials in neurons. Gold pellets have genes that you want to transfer; have dna coating them: rhisobium radiobacter is an organism that has a natural ability to infect the ge(cid:374)o(cid:373)e of pla(cid:374)ts. Take one of the transformed cells and deliver it to a fertilized mouse embryo; embryo develops into a mosaic. Put one plasmid/well; in one well is the fragment of dna that contains channelrhodopsin. Screen the wells using a pi(cid:374) (cid:396)epli(cid:272)ato(cid:396); goes i(cid:374) gel, the(cid:374) o(cid:374) a (cid:373)e(cid:373)(cid:271)(cid:396)a(cid:374)e p(cid:396)o(cid:271)e (cid:449)ill (cid:271)i(cid:374)d to (cid:272)ha(cid:374)(cid:374)el(cid:396)hodopsi(cid:374) gene and now you know which well contains it so you can amplify it. Incorporation of them into a growing strand causes elongation to be terminated. The shortest dna strand will travel farthest; whichever lane it is in tells you which nucleotide is first. I(cid:374) (cid:272)lassi(cid:272)al a(cid:374)ge(cid:396) se(cid:395)ue(cid:374)(cid:272)i(cid:374)g, the diffe(cid:396)e(cid:374)t dideo(cid:454)(cid:455)(cid:374)u(cid:272)leotides do(cid:374)"t ha(cid:448)e different colours. Repeats: crispr is a bacterial immune system, virus infects bacterium, dumps its.

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