Biology 1002B Study Guide - Midterm Guide: Enzyme Kinetics, Rubisco, Facilitated Diffusion
16 Mar 2017
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Change in respiration rate (oxygen consumption) in isolated mitochondria by addition of nadh, adp, uncoupler, etc: to measure respiration/the speed of etc, we measure the rate of oxygen consumption. Procedure: grind up chlamy cells, purify intact mitochondria, incubate in a buffer. Definition of respiratory control and how it is accomplished (proton gradient) Mitochondria: energy comes from oxidation of glucose: final electron acceptor is oxygen, produces carbon dioxide. Chloroplast: energy comes from light: final electron acceptor is nadp, produces oxygen (one of the substrates in mitochondria, atp produced does not leave the chloroplast, no atp transporter, and all atp is consumed in calvin cycle. Autotrophic: produce complex organic compounds from substances in surrounds (ie. light, inorganic chemicals) Heterotrophic: acquires energy and organic compounds from eating: cannot fix carbon from inorganic sources. Reasons why chlamy can grow as a heterotroph on some reduced carbon compounds but not others.
