Biology 1002B Study Guide - Midterm Guide: Electrochemical Gradient, Oxidative Phosphorylation, Atp Synthase

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peachwildebeest270

11 Feb 2018

School

Western University

Department

Biology

Course

Biology 1002B

Professor

Tom Haffie Denis Maxwell

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Document Summary

Correctly identify how changes to cellular respiration can shift metabolite ratios: blocking oxidative phosphorylation will lower the amount of nad+ that gets oxidized. Definition of oxidative phosphorylation: process to oxidize nadh (the substrate) to produce water and citrate, the oxidative part is the etc part, and the phosphorylation is the proton gradient part, happens in the inner mitochondrial membrane. How is the energy in nadh used in electron transport: the etc uses the energy from nadh to pump protons into the intermembrane space. Role of the proton gradient in chemiosmosis: the proton gradient is used to do work (to make atp, the head group of atp synthase spins and drives the force of atp using the proton gradient. Individual coupling: taking two different reactions and adding them together: coupling is important to carry out energetically unfavourable reactions (requires energy to work) If energy runs out, the reactions will stop and the cell will die.

Related Questions

Oxidative phosphorylation is the final stage of cellular respiration and the main pathway of aerobic energy generation from glucose. Which of the following statements is NOT true?

a. NADH and FADH2 are used as âinputâ into the electron transport chain

b. The electron transport chain produces a proton gradient across the inner mitochondrial membrane

c. FADH2 stores less energy than NADH

d. The electron transport chain is made up of several membrane protein complexes

e. ATP synthesis by chemiosmosis requires about 30 protons per ATP molecule

Oxidative phosphorylation questions - biochemistry (please be clear and don't copy/paste from google).

1. What is the function of a proton wire?

2. Describe Peter Mitchellâs mechanism for chemiosmotic coupling the energy of the transmembrane proton gradient to drive the endergonic process of ATP synthesis.

3. What transport proteins occur in the inner mitochondrial membrane? â¦.the outer mitochondrial membrane?

4. List the different types of redox centers in the respiratory electron transport chain. Which are one-electron carriers and which are two-electron carriers?

1. Compared to the generation of ATP by oxidative phosphorylation, what is a major difference in mechanism of ATP generation by substrate-level phosphorylation in glycolysis?

A. A proton gradient is not involved

B. The ATP produced has less energy content because oxygen was not involved.

C. Substrate level phosphorylation occurs in the inter-mitochondrial space

D. One can't generalize without knowing the substrate.

2. Which of the following statements is true?

A. Because the electrons in NADH are at a higher energy than the electrons in reduced ubiquinone, the NADH dehydrogenase complex can pump more protons than can the cytochrome b-c1 complex.

B.The pH in the mitochondrial matrix is higher than the pH in the intermembrane space.

C. The proton concentration gradient and the membrane potential across the inner mitochondrial membrane tend to work against each other in driving protons from the intermembrane space into the matrix.

D. The difference in proton concentration across the inner mitochondrial membrane has a much larger effect than the membrane potential on the total proton-motive force.

Biology 1002B Study Guide - Midterm Guide: Electrochemical Gradient, Oxidative Phosphorylation, Atp Synthase

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