Biology 1002B Study Guide - Citric Acid Cycle, Photosynthetic Reaction Centre, Opsin

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Energy point of view and information point of view. Energy fixation - occurs in the thylakoid membranes. Carbon fixation ( calvin cycle) - occurs in the stroma. There is less fluorescence in the cell sample because the cell requires a lot of energy for it to run the cell processes it needs to function properly. Therefore, the energy produced by the excitation could be used by essential molecules and cell organelles to perform these processes. The energy can also be transferred to the reaction center to drive photochemistry as part of photosynthesis. It is possible that the cell has developed an evolutionary mechanism(s) which help utilize light energy by minimizing energy loss and the amount of fluorescence given off. In the isolated chlorophyll, there is no pathway for the energy produced to be utilized so it is released via fluorescence.

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