Biology 3316A/B Study Guide - Final Guide: Peroxin-7, Senescence, Pex5
30 Mar 2015
School
Department
Course
Professor
Document Summary
Regulated cellular suicide mechanism characterized by nuclear condensation, cell shrinkage, membrane blebbing, and dna fragmentation. Video on how it looks: (on slide) Overview of birth, lineage, and death of cells. Three options for a cell: divide, differentiate, or die. Usually a signal for each pathway: if there is no signal present = cell death. Ultra-structural features of cell death by apoptosis (fig. ) Blebbing: protrusion, or bulge, of the pm, caused by decoupling of the cytoskeleton from the pm. Much of our knowledge comes from their study. Screening using nomarski dcm observed apoptosis genes in these nematodes. Ced-1 gene: mutations prevent engulfment of dead cells highly refractile dead cells accumulate, facilitating visualization (arrows in fig. a below indicate 3 highly refractile cells) Primary mediators: caspases family of aspartate-specific, cysteine proteases. Three types of mammalian caspases: apoptotic initiator caspases: activate effector caspases, are specific, apoptotic effector caspases: more general function, caspases involved in inflammatory cytokine not involved in apoptosis (just fyi)
