Physiology 3140A Study Guide - Quiz Guide: Intron, Agarose Gel Electrophoresis, Blastocyst
29 Dec 2016
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Can have tissue-speci c knockout isolate the gene production"s function within a particular tissue or cell type. Using a mouse as the model organism + knockout = knockout mouse: knock-in: the gene of interest is speci cally targeted into a genome to a precise location. Can have tissue-speci c knock-in isolate the gene production"s function within a particular tissue or cell type: over expression: adding an extra copy of the gene, resulting in the over production of gene product. A variation of the trasgeneotic model, created to identify how having extra copies affects the physiology of the organism. Could be incorporated into the genome randomly, location not speci ed (it"s a type of allele mutation) Note: knock-in is different from transgenic (over expression and dominant negative: transgenic mice are non-targeted, the gene of interest is introduced into the nucleus and selection is only based on random incorporation (location not speci ed)
