Physiology 3140A Study Guide - Quiz Guide: Pipette, Chemiluminescence, Long Term Ecological Research Network
29 Dec 2016
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Western blot: the analysis used to quantify protein expression, used to examine the effects of treatments (ex: broth factors & inhibitions) or used for gene manipulation on a cell population. Flowchart: protein isolation, from the sample (a whole tissue or cells in culture) If the protein of interest is intracellular = cells must be ruptured with a detergent = release proteins: protein quanti cation. The protein extracted must be equal in amount between different samples (that are going to be compared) total protein extracted must be quanti ed, identify units of protein (mg) in the volume of isolation buffer (ml) The collected protein samples must be quanti ed to ensure that equal amounts of total protein are loaded in each well = allows accurate comparisons between different experimental conditions. 5. transferred from the gel onto a membrane (which is more resilient than a gel)
