BI276 Study Guide - Final Guide: Bunsen Burner, Gram Staining, Negative Stain
Document Summary
Aseptic technique: working in an environment in a way that sterility is maintained. Sterilization: is the elimination of microbiological organisms to achieve a sterile microbial environment. Lab itself is not sterile, that is why a bunsen burner is used to push any airborne particles away from the. Open agar plates with lid slightly lifted near the bunsen burner. Open test tubes on a slant, flame the opening of each tube. Total magnification of an object is the result of two lenses working together: the ocular lens and the objective lens. Stains are organic compounds that bind to cellular components. Positively charged (basic dyes) bind strongly to negatively charged bacterial cell surfaces. If this stain stains a bacterial cell its called a direct stain. If this stain stains the background, the cell is called a negative stain. The gram stain is a type of differential stain that differentiates bacteria based on their cell wall properties.
