CH250 Study Guide - Midterm Guide: Elution, Titration, Column Chromatography

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9 Sep 2014
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General structure: a carboxyl group, an amino group, alpha carbon that has a chiral centre, the chiral centre makes them enantiomers. If arranged clockwise, d: all of the common amino acids are l amino acids, there is. Phosphorylation is the most common type of regulatory modifications: omithine and citrulline are not found in proteins but are intermediates for the biosynthesis of arginine and in the urea cycle. Peptides and proteins: peptides are polymers of amino acids, amino acids are joined together by a peptide bond (very stable) Prosthetic groups the non- amino acid part of a conjugated protein. Working with proteins: purficiation, first step is breaking open the cells containing the proteins and releasing their proteins into a solution called a crude extract. Commonly the extract is subjected to treatments that separate the proteins into different fractions: fractionation, separation based in the properties of the protein. Size, charge, shape, binding affinity, etc: column chromatography.