BIOL1000D Test 2 - Version A
1. You are completing VERSION A. Bubble in “A” beside #1 on your scantron. THIS QUESTION IS NOT
WORTH A MARK BUT NEEDS TO BE COMPLETED TO BE GRADED.
A. Pick this one!
2. You are studying a particular enzyme catalyzed reaction during one of your 4 year Biology lab
courses. This reaction has a G of -12 kcal/mol. If you were to double the amount of enzyme,
what will the G be for your reaction?
A. -12 kcal/mol
B. +12 kcal/mol
C. +24 kcal/mol
D. -24 kcal/mol
E. 0 kcal/mol
3. In the diagram below, solid arrows represent enzyme catalyzed reactions. The dashed arrow
represents molecule R interacting with an enzyme. If the enzyme catalyzing the N to Q reaction is
inhibited by product P what will the final products and relative amounts be if the reactions are
permitted to go to completion? (Assuming all enzymes have equal rates of activity).
A. N only
B. Q only
C. P and Q at first, then more Q
D. P and Q at first, then more P
E. N and Q in equal amounts
F. P and Q in equal amounts regardless of time
4. Which of the following best describes how an enzyme works?
A. They bind to a substrate and increase the activation energy
B. They convert endergonic reactions into exergonic reactions
C. They bring reactants together and lower the activation energy
D. They bind to a substrate and decrease the change in free energy
Page 1 of 9 BIOL1000D Test 2 - Version A
5. What is the difference between potential energy and free energy?
A. Potential energy refers to the energy stored in a system; free energy is the energy released
during an endergonic reaction.
B. Potential energy refers to the energy stored as the activation energy; free energy is the energy
remaining at the end of a reaction.
C. Potential energy refers to the energy stored in a system; free energy is the portion of the
system’s energy available to do work.
D. There is no difference; they mean the same thing.
6. The metabolic pathway in the figure below is
A. Anabolic; endergonic
B. Catabolic; endergonic
C. Anabolic; exergonic
D. Catabolic; exergonic
7. In the figure below, which of the following statements is true?
A. The reaction is considered to be endergonic.
B. The reaction will have a negative G value.
C. The free energy change of the reaction is indicated by arrow #2.
D. The free energy change of the reaction is indicated by arrow #3.
Page 2 of 9 BIOL1000D Test 2 - Version A
8. Succinate dehydrogenase catalyzes the conversion of succinate to fumarate. The reaction is
inhibited by malonic acid. Increasing the concentration of succinate relative to malonic acid
reduces the inhibitory effect of malonic acid. Based on this information, which of the following is
A. Succinate dehydrogenase is the enzyme, and fumarate is the substrate.
B. Fumarate is the product, and succinate is a competitive inhibitor.
C. Fumarate is the substrate and malonic acid is a competitive inhibitor.
D. Fumarate is the product, and malonic acid is a competitive inhibitor.
E. Succinate dehydrogenase is the enzyme, and malonic acid is the substrate.
9. What does it mean when an enzyme has an allosteric activator?
A. The allosteric activator will bind to the enzyme at a site other than the active site and inhibit
B. The allosteric activator will bind to the enzyme at the active site and stimulate enzyme activity.
C. The allosteric activator will bind to the enzyme at a site other than the active site and stimulate
D. The allosteric activator will bind to the enzyme at the active site and inhibit enzyme activity.
10. You are sitting with a friend and he/she asks you; “how can I increase my metabolic rate?”. You
immediately remember your Biol 1000 lecture and answer
A. decrease your muscle mass, and increase your fat content
B. increase your muscle mass and decrease your fat content
C. increase your internal temperature and increase your fat content
D. increase your internal temperature and stop eating
E. decrease your muscle mass and decrease your fat content
11. An animal cell is placed in a beaker of solution. The cell immediately begins to swell and
ultimately bursts. Why did this happen?
A. Because the cell’s cytoplasm was hypotonic relative to the solution and the solution was isotonic
relative to the cell cytosol.
B. Because the cell’s cytoplasm was hypertonic relative to the solution, and the solution was
hypotonic relative to the cell cytosol.
C. Because the cell’s cytoplasm was hypertonic relative to the solution, and the solution was
isotonic relative to the cell cytosol.
D. Because the cell’s cytoplasm was hypotonic relative to the solution, and the solution was
hypertonic relative to the cell cytosol.
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12. What is responsible for the selective permeability of a cell membrane?
A. The position of cholesterol in the membrane bilayer
B. The peripheral proteins of the membrane
C. The hydrophobic core formed by the phospholipid tails
D. The hydrophilic end facing the cell exterior
13. You have discovered a new ion pump that moves chloride into the cell (chloride concentrations
are lower outside the cell than inside the cell). The pump also hydrolyzes ATP. What have you
most likely discovered?
A. A primary active transport protein.
B. A secondary active transport protein.
C. An ion channel.
D. A peripheral protein that uses ATP to flip from one side of the membrane to the other when it
14. What two processes are involved when eukaryotic cells import and secrete large molecules?
A. Exocytosis when importing and diffusion when secreting
B. Exocytosis when importing and endocytosis when secreting
C. Endocytosis when importing and exocytosis when secreting
D. Diffusion when importing and exocytosis when secreting
15. What is the difference between simple and facilitated diffusion?
A. Facilitated diffusion requires energy whereas simple diffusion does not.
B. Facilitated diffusion requires a transport protein whereas simple diffusion not.
C. Facilitated diffusion moves a solute from an area of low concentration to high concentration
while simple diffusion moves a solute from an area of high concentration to low concentration.
D. Answers A and C.
16. The following represents an amino acid sequence for a protein.
Amino group-P-P-P-P-P-P-P-N-N-N-N-N-N-N-N-N-N-N-N-N-N-N-N-N-P-P-P-P-P-P-P-carboxyl group
N = Non-polar amino acid
P = Polar amino acid
Based on this sequence, this protein is most likely a
A. Peripheral membrane protein
B. Polytopic multipass membrane protein
C. A monotopic membrane protein
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17. Epinephrine is a signaling molecule (chemical messenger), that binds to a G-protein coupled
receptor and activates a cyclic AMP pathway. An inhibitor of phosphodiesterase activity would
have which of the following effects?
A. Block the response of epinephrine at the level of the G-protein coupled receptor