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Bio1000

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Department
Biology
Course
BIOL 1500
Professor
Tamara Kelly
Semester
Winter

Description
September 7th 2012 Cathy Nguyen=) Astrobiology Light - the portion of the electromagnetic spectrum that humans can detect with their eyes - categorized as a wave (stream of energy particles) - these particles are categorized as photons (visible light particle) - photons have no energy or mass but have precise amount of energy Ex. Blue light has a shorter wavelength = less photons = less energy than... red light Ex. Sunburn = human response to light, shows some physical manifestication on you to know that these particles exist Ex. Radiowaves = hearing noise is a direct result that it exists Photoreceptor: a basic light-sensing protein system invovled in sensing and responding to light (Contains a protein + pigment, the pigment absorbs light photon while the protein undergoes conformational change) Eyespot: a heavily pigmented region in certain one-celled organisms that function as a light sensor. Detects both direction & intensity of light Interactions with Matter 1. Reflect: bounces off and sent to another direction (occurs b/c frequencies of light waves don't match with the natural frequencies of vibration of the object, same with transmission) 2. Transmitted: passes through with no interaction 3. Absorbed: most useful for biological, can only use a photon when absorbed (ex. cup of water, can only be beneficial when we drink it) Pigments - a molecule that can absorb photons of light (they differ by different wavelengths each can absorb, depending on its structure) - its colour is the colour that was not absorbed, but reflected off - made from double/single bonding arrangement called conjugated system Ex. Chlorophyll Rhodopsin - a photoreceptor found universally in all organisms - technically, a pigment in the retina that allows the eye to see in dim light - made of a protein called opsin, that binds to a single pigment molecule called retinal In light: A photon is absorbed causing the retinal molecule to under go conformational change, which results in a whole bunch of other reactions that create electrical signals that are sent to the brain. Enzymes cause it to reset to original shape (conformational change). Light as a Source of Energy - when a photon of light is absorbed, an electron within the pigment molecule is raised to an excited state, which is a source of potential energy that can be put to work. This potential energy can be used in photosynthesis (electron transport chain to synthesize NADPH), when then create ATP>C02>CARBS. - some of the chemical energy is also used to synthesize other molecules like lipids, proteins, nucleic acids Sensing Light without Eyes - plants, invertebrates, & some prokaryotes don't have eyes but can sense light Ex. C. reinhardtii: its eyespot is located within the chloroplast in a region closely associated to the cell membrane. Although it is in the chloroplast, the eyespot does not play a role in photosynthesis but rather the photoreceptors of the eyespot allow the cell to sense light direction and intensity. (phototaxis) - in plants, a different receptor called the phytochrome senses the light environment and is critical for photomorphogenesis (development process when seedlings are exposed to light). Phototaxis (+/-): the ability of organisms to move directionally in response to a light source (maximizes light capture for photosynthesis). The Eye (the organ animals use to sense light) - requires a brain/simple nervous system to interpret signals sent from the eye. The eye&brain are thought to be co-evolved b/c detailed visual processing occurs in the brain rather than the eye. We essentially "see" with our brain and not our eyes. Ex. Planarians: ocellus (plural ocelli) is the simplest eye consisting of up to 100 photoreceptors lining in a cup/pit under the skin. Each ocellus is covered by a layer of pigment cells that block most of the light rays arriving from the opposite side of the animal. Resulting in most of the light recieved by the pigment cells to enter from the side it's facing. This way, planarians orient themselves so that the amount of light falling on the two ocelli is equal and deminishes as they swim. This reaction carries them directly away from the source of light and toward darker areas. Imaging-forming eyes Compound Eyes: common in insects and crustaceans, containing thousands of ommatidia (omma = eye) units fitted closely together. Each ommatidium samples a small part of the visual field as light enters. Signals from the photoreceptor cells go to the brain creating a mosaic image of the world. Even the slightest motion is detected. Single-lens Eyes: for most veterbrates, light enters the ee through a transparent cornea, a lens concentrates the light, and a layer of photoreceptors at the back of the eye (retina) records the image. Evolution of the Eye - scientists believe that about 2000 small improvements over time from a patch of light-sensitive cells, gradually yielded a camera-type eye in less than 1/2 a million years. 1) Region of photosensitive cells (just an eyespot, ex. Euglena) 2) Depressed/folded area allows limited directional sensitivity (eyecup, ex. Dugesia/Planarians) 3) "Pinhole" eye allows finer directional sensitivity and limited imaging (ex. Nautilus) 4) Transparent humour develops in enclosed chamber 5) Distinct lens develop (Primitive lens, ex. Box Jellyfish) 6) Iris and seperate cornea develop - looking at closely related organisms & how their eyes differ in relation to their environment. Ex. Limpet - simple layer of photosensitive cells Slit-shell Snail - simple layer of photosensitive cells curved in eyecup Nautilus - pinhole camera eye Squid - advanced camera eye September 10th 2012 Role of Light in Ecology & Behavior Light can Damage Biological Molecules: Direct Effects - light is a small portion of the electromagnetic spectrum et is essential to life on Earth. This is b/c it is teh most dominant form that reaches the surface. Shorter wavelengths are absorbed by the ozone layer high in the atmosphere whereas longer ones are absorbed by water vapour and CO2 in the atmosphere. - also b/c radiation of shorter wavelengths has high enough energy to destroy bonds of living things. It wouldn't just excite electrons but would oxidize the molecule producing ions (ionizing radiation). - wavelengths that are longer don't supply enough energy to excite electrons for photochemistry. Longer wavelengths are also readily absorbed by water (the bulk of living things). - Light is still damaging, but all organisms that are exposed to light have developed mechanisms to help prevent damage/repair quickly if damage does occur. Ex. Photosynthesis: composed of photosystems (pigment-protein complexes) that trap photons and convert it to chemical energy. Although very efficient, the high-energy environment does cause damage to the protein complexes. Under high light conditions, carotenoids (accessory pigments) protect the photosynthetic apparatus from high light levels by absorbing exess light and dissipating the energy as heat. Light can Damage Biological Molecules: Indirect Effects - harmful indirectly b/c of UV rays (ultraviolet radiation). Life on Earth is protected by the most damaging, UV-C by the ozone layer, however UV-A and UV-B can still reach the surface. B/c of its high energy, it can randomly ionize atoms in pigment molecules and proteins. - DNA is very vulnerable to UV damage b/c of its structure, resulting in the formation of a "dimer" when two neighboring bases become linked. This changes the shape of the double-helix and prevents replication, hindering gene expression (genetic mutation). Ex. Animals avoid sunlight by shielding skin with fur/feathers, whereas humans rely on melanin. Melanin: pigment that absorbs UV radiation, preventing it from penetrating the skin and destroying the essential B vitamin folate. Absorption Spectrum: plot of the amount of light a pigment absorbs in relation to the wavelength of light. - humans synthesize melanin in skin cells called melanocytes. People from countries recieving a lot of sunlight (like Uganda) have more melanin than ppl with less sunlight (Swedan). - humans need some UV rays to synthesize vitamin D, which is critical for bone development. Ppl with high melanin levels who live in regions that don't receive abundant sunlight are susceptable to Vitamin D deficiency, ex africans living in swedan. (rare b/c many foods are fortified with vitamin d) - native americans/inuits have relatively dark skin even tho they live in nowhereland b/c they mainly eat fish and marine life whic his naturally high in vitamin D. Circadian Rhythms - the physiological and behavioral responses geared to Earth's day-night cycle (24 hr day). - not direct responses to change in the external light environment but are controlled by an internal clock. - found in the suprachiasmatic nucleus, a region within the hypothalamus. It recieves light inputs directly from the optic nerve, which uses it to set the biological clock. This regulates body functions such as secretin of melatonin (only made @ night and inhibited during the day) Ex. Jet lag: when you travel across many time zones, putting hte circadian clock out of sync. Ex. Birds hibernating south during winter & returning in Spring. - outside factors come into play as well, like weather and noise. - provides a lot of information about how an organism works, how efficient it is, etc. Ex. Plants make all prosynthetic enzymes at night so they can absorb the most energy during the day. Ex. Humans do a lot of DNA synthesis at night, b/c during the day we are getting hit by UV rays. Ex. Blind mole rat: can still have a biological clock b/c photoreceptors can still sense light but can't create the image. Ex. Alaska - artificial light system - animals that live in cages need some type of regulation for their biological clock (under investigation) Camouflage - disguising from predators, depending on the perception of the predator. - must be indistinguisable in order to be useful Ex. Pepper Moth: trees got covered by slick, therefore dark moths lived and light moths got killed. Then, pollution laws came into play and vice versa occured. Ex. Skunk warning, monarch buttery eaten = tastes nasty. Plants = pretty to attract pollenators (Red/yellow for birds, small white/green & no odor for bee. Not all colours of light spectrum are visible to certain species, therefore UV markings create a "bullseye." Bees have adapted to see this marking. Silver bellies/black backs for fish, therefore predators below will think they're just look at the surface, vice versa. September 12th 2012 Biodiversity Biodiversity: reflects the reality that life on Earth exists from the ocean floor to wel above the atmosphere, the study of biology focuses on all levels of life Cell: smallest unit with the capacity of live and reproduce, independentl or part of a multicellular organism. Must be able to grow, divide and respond to outside stimulus (ex. circadian) Multicellular Organism: individual consisiting of interdependent cells Population: Group of individuas of the same species that occupy the same area Community: Population of all species that occupy the same area Ecosystem: Group of communities interacting with their shared physical environment. Linked by nutrient cycles, energy flow (how they get energy?) Biosphere: All regions of the Earth that can sustain life How Organisms Obtain Carbon - carbon is the backbone of all organic molecules synthesized by an organism Autotroph (most plants): self-nourishing, synthesize organic carbon using inorganic carbon (CO2). Although CO2 conta
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